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AutoXpress® (AXP)

The Best in Cellular Processing & Verifiable Tracking. Test it for yourself – On Us!

The AXP® is an automated, functionally closed system that consistently and efficiently harvests the stem cell-rich buffy coat from umbilical cord blood.

The system reduces a unit of cord blood to a precise volume selected by the operator and does so with the highest precision, safety, and confidence.

  • Automates the volume reduction process
  • Provides consistent buffy coat concentration volumes
  • Ensures high recoveries of mononuclear cells (MNCs) in a targeted volume1
  • Allows for simultaneously processesing of multiple cord blood units in one centrifuge

Complete System Solution.

The AXP® System includes:

  • AXP device
  • Docking station
  • Processing set
  • XpressTRAK® software
  • Accessories

Highest Yield. Viable Cells.

  • Precise delivery of final volume target1
  • Reliable collection of all fractions: buffy coat, RBC, and plasma
  • Viable CD34+ stem cell recovery of >97% is higher than other available systems2
  • Cord blood units (CBU) processed with AXP are richer in mononuclear cells (MNC) than units conventionally processed having similar total nucleated cells (TNC)1
  • Hematocrit is consistently <30% in final storage volume.1

Uncompromising Safety. Quality Results.

  • Utilizes a functionally closed processing set
  • Separate collection bags for capturing buffy coat, plasma, and RBC
  • Integrated sampling pillows for pre- and post-processing sterile sample collection
  • Transferring cord blood from collection bag can be done by:
    • sterile weld
    • spike port
  • In-line clot filter and multiple sampling ports
  • Integrated DMSO inlet filter and tubing

Efficient Automation. Integrated Quality Control.

  • Xpress TRAK® software tracks and documents data specific to each cord blood unit processed
  • Captures sample processing data to assist with quality assurance and compliance with current good tissue practices (cGTP) and good manufacturing practices (cGMP)
  • Stores processing data in a searchable, sortable database
  • Customizable data fields and reports
  • Optional XpressLINK software allows for networking multiple computer workstations

Docking Station

  • Recharges the device battery
  • Provides the communication link between the AXP device and the computer workstation that has the Xpress TRAK software

1. Data on file.

2. Rubenstein P. Cord blood banking for clinical transplantation. Bone Marrow Transplantation 2009; 44:635-642.

Cell Separation

AXP® System is a fully automated, programmable cell separation and recovery system, utilizing microprocessors and flow controlled optical sensors. The AXP System requires no manual processing and is designed to reduce human error and deliver consistent, reproducible results.

AXP directs cord blood components (buffy coat, plasma, red blood cells) into separate sterile bags. The buffy coat volume is defined by the user and is captured in a freezing bag which is ideal for cryopreservation and use in transplantation. Accurate and reproducible spin speeds, acceleration and G forces ensures accurate processing without damage to the cells.


Cord blood is transferred into the processing bagset which is placed in the AXP device and then centrifuged. The AXP device fits into most standard blood bank centrifuge buckets allowing up to six samples to be processed simultaneously. During centrifugation, component stratification and separation occurs. Red blood cells (RBC) are transferred to a separate sterile bag, the buffy coat which contains the mononuclear cell (MNC) rich layer is delivered to a separate sterile freezing bag while the plasma remains in the processing bag. The AXP system does not require the addition of hetastarch for successful capturing of MNC’s.

AXP® System: Processing Steps and Harvesting Cord Blood

  1. Transfer the cord blood to the AXP processing set and collect a pre-processing sample using the sampling pillow.
  2. Load the processing set into the AXP device.
  3. Spin up to six AXP devices in the centrifuge to separate and harvest the stem-cell rich blood fraction.

  1. Remove the processing set.
  2. Place the AXP device into the docking station.
  3. Processing data is automatically downloaded and captured in the XpressTrak software.
  4. Collect a post-processing sample using the sampling pillow, add cryoprotectant to the buffy coat, seal the freezing bag and place sample in an overwrap bag and canister for storage in the BioArchive® cryopreservation system.

Recovery Rates3

The following table represents the typical recovery rates users can expect from the AXP System.

Cell Population Pre-Process
(Cord Blood)
(Harvest MNC Fraction)
% Recovery
Total Nucleated Cells x 106 1150 ± 273.4 969 ± 225.3 84.8 ± 9.2
Total Mononuclear Cells x 106 453 ± 134.6 442 ± 126.6 97.9 ± 4.9
Total CD34+ Cells x 103
3364 ± 1594.0
(99.7 ± .03)
3256 ± 1412.7
(99.8 ± .02)
98.2 ± 8.0
CFU x 106 2.6 ± 1.1 2.5 ± 1.0 94.6 ± 7.0

Processing of Cord Blood

Filled processing/plasma bag prior to centrifugation


AXP Centrifugation: High Speed Spin

  • 1,400 RCF
  • Stratification of blood components


  • Cell stratification complete after 20 minutes


AXP Centrifugation: Low Speed Spin

  • 80 RCF
  • RBC Removal


  • Harvest stem cells (20-22 ml’s)


3. Data was provided by New York Blood Center from a study performed using 20 CBUs processed without HES

Published Journal Articles

    • Rubinstein, P. Cord blood banking for clinical transplantation. Bone Marrow Transplantation (2009) 44, 635–642; doi:10.1038/bmt.2009.281; published online 5 October 2009.
    • Solves P, Mirabet V , Blanquer A , Delgado-Rosas F , Planelles D, Andrade M , Carbonell-Uberos F , Soler MA and R Roig. A new automatic device for routine cord blood banking: critical analysis of different volume reduction methodologies. Cytotherapy December 2009, Vol. 11, No. 8 , Pages 1101-1107. Read More:
    • Takanashi M, Oba A, Ogawa A, Ito M, Kawahata Y and K Nakajima. Red blood cell depletion of cord blood using an automated system-Evaluation of the AXP system. Japanese Journal of Transfusion and Cell Therapy, Vol. 56. No. 1 56(1) : 62-67, 2010.
    • Solves, P., et al. (2013). Qualitative and quantitative cell recovery in umbilical cord blood processed by two automated devices in routine cord blood banking: a comparative study.  Blood Transfus 11(3): 405-411.
    • Dobrila L, Jiang S, Chapman J, Marr D, Kryston K and P Rubinstein. Thermogenesis AXP AutoXpress Platform and BioArchive System for Automated Cord Blood Banking. BMT Tandem Meeting Abstract 2006.
    • Roh, EY., Et al. Cord Blood Volume Reduction Using the Automated Devices Sepax(R) S-100 and AXP(TM) AutoXpress Platform. Korean J Blood Transfus. 2007 Dec 18 (3):219-226. Korean.
    • Harris, D. Collection, processing, and banking of umbilical cord blood stem cells for clinical use in transplantation and regenerative medicine. March 2008. Volume 39 Number 3. Labmedicine.
    • Willert, J., et at. Umbilical cord blood- biology, banking and therapeutic use. Neonatal Medicine. US Obstretrics & Gynecology 2008, 68-72.
    • Badowski, M. S. and D. T. Harris (2012). Collection, processing, and banking of umbilical cord blood stem cells for transplantation and regenerative medicine.  Methods Mol Biol 879: 279-290.


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